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thp1 dual tlr4 md  (InvivoGen)


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    InvivoGen thp1 dual tlr4 md
    Thp1 Dual Tlr4 Md, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1+dual+tlr4+md/THP1-Dual+MD2-CD14-TLR4+Cells/pm39950821-404-0-3
    Average 94 stars, based on 7 article reviews
    thp1 dual tlr4 md - by Bioz Stars, 2026-10
    94/100 stars

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    Cell Culture:

    Article Title: Discovery of Novel Isoxazole-Based Small-Molecule Toll-Like Receptor 8 Antagonists
    Article Snippet: Human embryonic Kidney (HEK)-Blue Null1, HEK-Blue hTLR2-TLR1, HEK-Blue hTLR2-TLR6, HEK-Blue hTLR4, HEK-Blue hTLR5, HEK-Blue hTLR7, HEK-Blue hTLR8, and HEK-Blue hTLR9 (InvivoGen, Toulouse, France) were cultured in Dulbecco’s modified Eagle’s medium (PAN-Biotech, Aidenbach, Germany) containing 10% (v/v) heat inactivated fetal bovine serum (FBS; S0615), 100 U/mL penicillin, 100 mg/mL streptomycin (P4333), 2 mM 1-glutamine (G7513) (all from Sigma-Aldrich, Taufkirchen, Germany), 100 μg/mL Normocin and selective antibiotics HEK-Blue Selection (hTLR2-TLR1, hTLR2-TLR6, hTLR4), 100 μg/mL zeocin (Null1), 100 μg/mL zeocin with 10 μg/mL (hTLR7, hTLR9), and 30 μg/mL (hTLR5, hTLR8) blasticidin (all from InvivoGen, Toulouse, France). .. THP1-Dual TLR4/MD-2/CD14 cells (InvivoGen, Toulouse, France) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), penicillin (100U/mL), streptomycin (100 μg/mL), l -glutamine (2 mM), HEPES (25 mM), Normocin (100 μg/mL), and selective antibiotics (blasticidin: 10 μg/mL, zeocin: 100 μg/mL) following the manufacturer’s instructions. .. THP-1 cells (ACC 16, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) were cultured in RPMI 1640 (11530586, Fisher scientific, Schwerte, Germany) containing 100 U/mL penicillin, 100 μg/mL streptomycin (P4333), 2 mM l -glutamine (G7513, both from Sigma-Aldrich, Taufkirchen, Germany), and 10% heat-inactivated fetal bovine serum (FBS; S0615, Sigma- Aldrich, Taufkirchen, Germany) at a density of 4 × 10 5 cells/mL to 2 × 10 6 cells/mL.

    Article Title: Label-free biosensor assay decodes the dynamics of Toll-like receptor signaling
    Article Snippet: THP-1 cells (passage 4–25) (#ACC 16, DSMZ, Braunschweig, Germany) were cultured in RPMI 1640 supplemented with 10% FCS, l-glutamine (2 mM), 100 U/ml penicillin, and 100 μg/ml streptomycin as described previously , . .. THP1-Dual TLR4/MD-2/CD14 (#thpd-mctlr4) and THP1-Dual KO-TLR4/MD-2/CD14 (#thpd-mckotlr4) cells (InvivoGen, Toulouse, France) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/ml), streptomycin (100 μg/ml), L-glutamine (2 mM), HEPES (25 mM), normocin (100 μg/ml) and the selective antibiotics blasticidin (10 μg/ml) and zeocin (100 μg/ml) following the manufacturer’s instructions. ..

    Article Title: Discovery of Novel Isoxazole-Based Small-Molecule Toll-Like Receptor 8 Antagonists.
    Article Snippet: Toll-like receptor 8 (TLR8) recognizes viral and bacterial RNA, initiating inflammatory responses that are crucial for innate immunity.. Dysregulated TLR8 signaling contributes to autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis, driving chronic inflammation and tissue damage.. Therefore, targeting TLR8 has gained attention as a promising therapeutic strategy.

    96 Well Plates:

    Article Title: Discovery of Novel Isoxazole-Based Small-Molecule Toll-Like Receptor 8 Antagonists
    Article Snippet: After 24 h, NF-κB activity was measured using QuantiBlue solution (InvivoGen, Toulouse, France) following the manufacturer’s instructions. .. THP1-Dual TLR4/MD-2/CD14 cells (InvivoGen, Toulouse, France) were seeded in 96-well plates at a density of 1 × 10 5 cells per well and were immediately preincubated with designated antagonists for 1 h and afterward stimulated with TLR agonists. .. After 24 h, NF-κB activity was measured via the SEAP reporter assay using QuantiBlue solution (InvivoGen, Toulouse, France) and ISRE activity was measured via lucia luciferase using QuantiLuc solution (InvivoGen, Toulouse, France), both following the manufacturer’s instructions.



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    Transfer of old microbiota enhances inflammatory bacterial components in serum. Human Embryonic Kidney 293 cells transfected with mouse toll-like receptor <t>(TLR)2/CD14</t> (A) or mouse <t>TLR4/MD-2/CD14</t> (B) were stimulated with 2.5% serum from young or old conventional (conv) mice ( n = 10), germ-free (GF) recipient mice of young or old microbiota ( n = 10), and GF control mice ( n = 5). Activation of these receptors was measured with a secreted embryonic alkaline phosphatase reporter coupled to the NF-kB/AP-1 promoter. All data are expressed as means. ** P < 0.01.
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    Transfer of old microbiota enhances inflammatory bacterial components in serum. Human Embryonic Kidney 293 cells transfected with mouse toll-like receptor (TLR)2/CD14 (A) or mouse TLR4/MD-2/CD14 (B) were stimulated with 2.5% serum from young or old conventional (conv) mice ( n = 10), germ-free (GF) recipient mice of young or old microbiota ( n = 10), and GF control mice ( n = 5). Activation of these receptors was measured with a secreted embryonic alkaline phosphatase reporter coupled to the NF-kB/AP-1 promoter. All data are expressed as means. ** P < 0.01.

    Journal: Frontiers in Immunology

    Article Title: Aged Gut Microbiota Contributes to Systemical Inflammaging after Transfer to Germ-Free Mice

    doi: 10.3389/fimmu.2017.01385

    Figure Lengend Snippet: Transfer of old microbiota enhances inflammatory bacterial components in serum. Human Embryonic Kidney 293 cells transfected with mouse toll-like receptor (TLR)2/CD14 (A) or mouse TLR4/MD-2/CD14 (B) were stimulated with 2.5% serum from young or old conventional (conv) mice ( n = 10), germ-free (GF) recipient mice of young or old microbiota ( n = 10), and GF control mice ( n = 5). Activation of these receptors was measured with a secreted embryonic alkaline phosphatase reporter coupled to the NF-kB/AP-1 promoter. All data are expressed as means. ** P < 0.01.

    Article Snippet: Human Embryonic Kidney 293 cells stably transfected with mouse TLR2/CD14 or TLR4/MD-2/CD14 and the secreted embryonic alkaline phosphatase reporter coupled to the NF-kB/AP-1 promoter were purchased from Invivogen (San Diego, CA, USA).

    Techniques: Transfection, Activation Assay